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KMID : 0364820170530020097
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Korean Journal of Microbiology
2017 Volume.53 No. 2 p.97 ~ p.102
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Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W
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Kim Kyoung-Ja
Jang Ju-Ho
Yang Yong-Joon
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Abstract
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Catechol type siderophore different from 2, 3-dihydroxybenzoic acid (DHB) was produced from Acinetobacter sp. B-W grown in medium containing L-glutamic acid as both carbon and nitrogen sole sources at 28°C. Optimal concentration of glutamic acid for siderophore production was 3% and production of siderophore was decreased above 3% glutamic acid. In previous report, siderophore, 2, 3-DHB was produced from strain B-W grown in medium containing glucose as carbon source and glutamic acid as nitrogen source. Rf value of siderophore produced from strain B-W grown in medium glutamic acid as both carbon and nitrogen sole sources at 28°C was 0.32, while 2, 3-DHB was 0.84 in butanol-acetic acid-water (12:3:5) as developing solvent. Antioxidative activity of 2, 3-DHB was not detected in that siderophore produced from glutamic acid. Catechol nature of siderophore was detected by Arnow test. Although in iron-limited media optimal cell growth was identified at 36°C, significant quantities of siderophore were produced only at 28°C. Biosynthesis of siderophore was strongly inhibited by growth at 36°C. Production of siderophore was completely inhibited by 10 μM FeCl3.
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KEYWORD
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Acinetobacter sp. B-W, L-glutamic acid, siderophore, sole carbon and nitrogen sources
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